Internal Control for Quality Assurance of Diagnostic RT-PCR
نویسندگان
چکیده
منابع مشابه
Internal control for quality assurance of diagnostic RT-PCR.
Reverse transcription polymerase chain reaction (RT-PCR) is gaining major importance in the field of medical diagnostics. For example, RT-PCR assays are being tested to become standard methodology for detection of occult tumor cells in peripheral blood of cancer patients. However, there is heterogeneity in the frequency of tumor cell detection, e.g., in melanoma patients as reported in the lite...
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The explosive increase since the beginning of 1990s in the number of publications reporting PCR-based methods for detection or molecular typing of food-borne pathogens has attracted the attention of end user laboratories. However, the well-recognized difficulties in reproducing published tests due to variation in the performance of PCR thermal cyclers (7) and in efficiencies of different DNA po...
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One approach to quantitate RNA is the use of a known number of competitive internal standard molecules that are reverse-transcribed and subsequently co-amplified under the same reaction conditions as the target sequence (3). This technique, called competitive reverse transcription polymerase chain reaction (cRT-PCR) has been applied to monitor the level of hepatitis C virus (HCV) RNA in patient...
متن کاملمعرفی و کلون سازی یک قطعه dna به عنوان یک کنترل داخلی (internal control) جهت تست rt-pcr رقابتی
نوویروس ها (nov) یکی از شایع ترین عوامل ایجاد کننده عفونت های غیرباکتریائی گاستروانتریت مزمن در جوامع انسانی می باشند. این ویروس ها اولین بار در سال1968 از اولین شیوع مربوط به مدرسه ای در منطقهnorwalk ایالت ohio آمریکا شناسائی گردیدند. نورویروس ها بسیار واگیردار بوده و به راحتی از طریق مدفوعی دهانی از فردی به فرد دیگر منتقل می شوند. بیماری، همراه با اسهال استفراغ بوده و 24-72 ادامه می یابد. این...
15 صفحه اولDiagnostic Ultrasound Scanner Quality Assurance
Medical Physics at CancerCare Manitoba has developed its own ultrasound phantom. The following table is intended as a quick reference guide when performing the quality assurance measurements. Instructions Limits Record Items Required ultrasound contrast-detail phantom degassed water or coupling gel paper towels for clean up (optional) lab stand and clamp to hold probe Physical check: 1. probe s...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98254dt04